Core Unit Metabolomics
Head: Dr. Almut Schulze
The aim of the core unit Cancer Metabolomics is to analyse changes in cellular metabolism in cancer cells. The unit is developing suitable technologies to accurately determine metabolite concentration in cancer cell lines and tumour samples by mass spectrometry. We are also establishing protocols for the labelling of cancer cells with stable isotope tracers (i.e. glucose or glutamine) for isotopomer spectral analysis (ISA) and metabolic flux analysis (MFA). These techniques allow the identification of metabolic intermediates derived from different nutrients and can reveal altered metabolic flux in cancer cells. In addition, the facility has instrumentation to determine oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of cancer cells in real time.
Lewis, C.A.*, Brault, C.*, Peck B., Bensaad K., Griffiths, B., Mitter, R., Chakravarty, P., East, P., Dankworth, B., Alibhai, D., Harris, A.L. and Schulze A. (2015) SREBP maintains lipid biosynthesis and viability of cancer cells under lipid and oxygen deprived conditions and defines a gene signature associated with poor survival in glioblastoma multiforme. Oncogene 34(40):5128-40, *equal contribution
Schug, Z.T.*, Peck, B.*, Jones, D.T., Zhang, Q., Grosskurth, S., Alam, I.S., Goodwin, L.M., Smethurst, E., Mason, S., Blyth, K., McGarry, L., James, D., Shanks, E., Kalna, G., Saunders, B., Jiang, M., Howell, M., Lassailly, F., Zaw Thin, M., Spencer-Dene, B., Stamp, G., van den Broek, N., Mackay, G., Bulusu, V., Kamphorst, J.J., Tardito, S., Strachan, D., Harris, A.L., Aboagye, E.O., Critchlow, S.E., Wakelam, M.J.O., Schulze, A. and Gottlieb, E. (2015) Acetyl-CoA Synthetase 2 Promotes Acetate Utilization and Maintains Cancer Cell Growth Under Metabolic Stress. Cancer Cell 27: 57-71, *equal contribution
Bensaad, K., Favaro, E., Lewis, C.A., Peck, B., Lord, S., Collins, J.M., Pinnick, K.E., Wigfield, S., Buffa, F.M., Li, J.L., Zhang, Q., Wakelam, M.J., Karpe, F., Schulze, A., Harris, A.L. (2014) Fatty acid uptake and lipid storage induced by HIF-1alpha contribute to cell growth and survival after hypoxia-reoxygenation. Cell Rep 9: 349-365
Ros, S.*, Santos, C.R.*, Moço, S., Baenke, F., Kelly, G., Howell, M., Zamboni, N. and Schulze, A. (2012) Functional screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB4) as an important regulator of prostate cancer cell survival. Cancer Discovery 2:328-343, *equal contribution
Porstmann, T., Santos, C.R, Griffiths, B., Cully, M., Wu, M. Leevers, S. Griffiths, J.R., Chung, Y.L. and Schulze A. (2008). SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth. Cell Metabolism, 8(3): 224-236.