piwik-script

Intern
    Onkologische Forschung

    Core Unit Metabolomics

    Summary

    Head: Prof Dr. Almut Schulze

    The aim of the core unit Cancer Metabolomics is to analyse changes in cellular metabolism in cancer cells. The unit is developing suitable technologies to accurately determine metabolite concentration in cancer cell lines and tumour samples by mass spectrometry (LC/MS, Thermo Fisher Q-Exactive Orbitrap). We are also establishing protocols for the labelling of cancer cells with stable isotope tracers (i.e. glucose or glutamine) for isotopomer spectral analysis (ISA) and metabolic flux analysis (MFA). These techniques allow the identification of metabolic intermediates derived from different nutrients and can reveal altered metabolic flux in cancer cells. In addition, the facility has instrumentation to determine oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of cancer cells in real time.

     

    Recent Publications

    Miess, H.*, Dankworth, B.*, Gouw, AM., Rosenfeldt, M., Schmitz, W., Jiang, M., Saunders, B., Howell, M., Downward, J., Felsher, DW., Peck, B. and Schulze, A. (2018) The glutathione redox system is essential to prevent ferroptosis caused by impaired lipid metabolism in clear cell renal cell carcinoma, Oncogene 2018 (in press) *equal contribution

    Snaebjornsson MT, Schulze A. (2018) Non-canonical functions of enzymes facilitate cross-talk between cell metabolic and regulatory pathways, Exp Mol Med. 2018 Apr 16;50(4):34, doi: 10.1038/s12276-018-0065-6.

    Klein-Hessling, S., Muhammad, K., Klein, M., Pusch, T., Rudolf, R., Floter, J., Qureischi, M., Beilhack, A., Vaeth, M., Kummerow, C., Backes, C., Schoppmeyer, R., Hahn, U., Hoth, M., Bopp, T., Berberich-Siebelt, F., Patra, A., Avots, A., Muller, N., Schulze, A. & Serfling, E. (2017) NFATc1 controls the cytotoxicity of CD8+ T cells, Nature Communication 8, 511.

    Ros, S., Flöter, J., Kaymak, I., Da Costa, C., Houddane, A., Dubuis, S., Griffiths, B., Mitter, R., Walz, S., Blake, S., Behrens, A., Brindle, KM., Zamboni, N., Rider, M.H. and Schulze, A., (2017) 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells, Oncogene, doi: 10.1038/onc.2016.477

    Rohrig, F., and Schulze, A. (2016). The multifaceted roles of fatty acid synthesis in cancer. Nat Rev Cancer.

    Schug, Z.T.*, Peck, B.*, Jones, D.T., Zhang, Q., Grosskurth, S., Alam, I.S., Goodwin, L.M., Smethurst, E., Mason, S., Blyth, K., McGarry, L., James, D., Shanks, E., Kalna, G., Saunders, B., Jiang, M., Howell, M., Lassailly, F., Zaw Thin, M., Spencer-Dene, B., Stamp, G., van den Broek, N., Mackay, G., Bulusu, V., Kamphorst, J.J., Tardito, S., Strachan, D., Harris, A.L., Aboagye, E.O., Critchlow, S.E., Wakelam, M.J.O., Schulze, A. and Gottlieb, E. (2015) Acetyl-CoA Synthetase 2 Promotes Acetate Utilization and Maintains Cancer Cell Growth Under Metabolic Stress. Cancer Cell 27: 57-71, *equal contribution

    Kontakt

    Comprehensive Cancer Center Mainfranken
    Haus C16
    Josef-Schneider Str. 6
    97080 Würzburg

    Tel.: +49 931 201-35350
    Fax: +49 931 201-35359
    E-Mail

    Suche Ansprechpartner

    Campus Medizin