Intern
    Onkologische Forschung

    Core Unit Metabolomics

    Summary

    Head: Dr. Almut Schulze

    The aim of the core unit Cancer Metabolomics is to analyse changes in cellular metabolism in cancer cells. The unit is developing suitable technologies to accurately determine metabolite concentration in cancer cell lines and tumour samples by mass spectrometry. We are also establishing protocols for the labelling of cancer cells with stable isotope tracers (i.e. glucose or glutamine) for isotopomer spectral analysis (ISA) and metabolic flux analysis (MFA). These techniques allow the identification of metabolic intermediates derived from different nutrients and can reveal altered metabolic flux in cancer cells. In addition, the facility has instrumentation to determine oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of cancer cells in real time.

    Recent Publications

    Lewis, C.A.*, Brault, C.*, Peck B., Bensaad K., Griffiths, B., Mitter, R., Chakravarty, P., East, P., Dankworth, B., Alibhai, D., Harris, A.L. and Schulze A. (2015) SREBP maintains lipid biosynthesis and viability of cancer cells under lipid and oxygen deprived conditions and defines a gene signature associated with poor survival in glioblastoma multiforme. Oncogene 34(40):5128-40, *equal contribution

    Schug, Z.T.*, Peck, B.*, Jones, D.T., Zhang, Q., Grosskurth, S., Alam, I.S., Goodwin, L.M., Smethurst, E., Mason, S., Blyth, K., McGarry, L., James, D., Shanks, E., Kalna, G., Saunders, B., Jiang, M., Howell, M., Lassailly, F., Zaw Thin, M., Spencer-Dene, B., Stamp, G., van den Broek, N., Mackay, G., Bulusu, V., Kamphorst, J.J., Tardito, S., Strachan, D., Harris, A.L., Aboagye, E.O., Critchlow, S.E., Wakelam, M.J.O., Schulze, A. and Gottlieb, E. (2015) Acetyl-CoA Synthetase 2 Promotes Acetate Utilization and Maintains Cancer Cell Growth Under Metabolic Stress. Cancer Cell 27: 57-71, *equal contribution

    Bensaad, K., Favaro, E., Lewis, C.A., Peck, B., Lord, S., Collins, J.M., Pinnick, K.E., Wigfield, S., Buffa, F.M., Li, J.L., Zhang, Q., Wakelam, M.J., Karpe, F., Schulze, A., Harris, A.L. (2014) Fatty acid uptake and lipid storage induced by HIF-1alpha contribute to cell growth and survival after hypoxia-reoxygenation. Cell Rep 9: 349-365

    Ros, S.*, Santos, C.R.*, Moço, S., Baenke, F., Kelly, G., Howell, M., Zamboni, N. and Schulze, A. (2012) Functional screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB4) as an important regulator of prostate cancer cell survival. Cancer Discovery 2:328-343, *equal contribution

    Porstmann, T., Santos, C.R, Griffiths, B., Cully, M., Wu, M. Leevers, S. Griffiths, J.R., Chung, Y.L. and Schulze A. (2008). SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth. Cell Metabolism, 8(3): 224-236.

    Kontakt

    Comprehensive Cancer Center
    Haus C16
    Josef-Schneider Str. 6
    97080 Würzburg

    Tel.: +49 931 201-35350
    Fax: +49 931 201-35359
    E-Mail

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